Structural Analysis of Labeled N-Glycans from Proteins by LC-MS/MS Separated Using a Novel Mixed-Mode Stationary Phase
19 Dec 2014
This application note describes the liquid chromatography-mass spectrometry (LC/MS) analysis of fluorescently labeled N-glycans released from proteins. The chromatographic separation is carried out with a novel Thermo Scientific™ GlycanPac™ AXH-1 column for high-resolution and high-throughput analysis of glycans. This column possesses unique selectivity that provides separation of glycans based on charge, size, and polarity.