UV Fluorescence Polarization as a Means to Investigate Protein Conformational and Mass Change Using Intrinsic Tryptophan Fluorescence in Conjunction with UV-capable Polarizers
14 Oct 2014

The essential amino acid tryptophan is intrinsically fluorescent with excitation in the UV range of the spectrum. This physical characteristic can be used to investigate peptides and proteins that contain this amino acid. This application note describes the use of the Synergy H1 reader in conjunction with UV-capable fluorescence polarizers to investigate conformational and mass changes of proteins in solution.

Show    per page
3 of 5     Page  1 2 3  ... Next 
Automated 384-Well Cell-Based Cytochrome P450 Inhibition Assays Using Cryopreserved Human Hepatocytes in Suspension CYP, toxicology, drug metabolism8 Apr 2014Read
Multiplexed Assay for IL-6 Secretion and Cell Viability using an Epithelial Ovarian Cancer Cell Line ovarian cancer7 Apr 2014Read
The Impact of a 3-Dimensional Human Liver Microtissue Model on Long-term Hepatotoxicity Studies microtissue, hepatotoxicity1 Apr 2014Read
BRD4 Bromodomain 1 Inhibitor Screening using a Homogeneous Proximity Assay histone modification, post-translational modifications1 Apr 2014Read
Interrogation of SET7/9 Histone Lysine Methyltransferase Using a High Throughput Fluorescence Polarization Screening Assay histone modification1 Apr 2014Read
Automated Fluorescence Detection and Imaging of RNA Species in Live Cells fluorescence, fluorescence based assay, RNA26 Mar 2014Read
Validation of a Novel Tumoroid-Based Cell Culture Model to Perform 3D in vitro Cell Signaling Analyses cell analysis, kinase assays25 Mar 2014Read
Incorporation of a Novel 3D Cell Culture System to Perform in vitro Cytotoxicity Analyses using Human Primary Hepatocytes 3d cell culture, apoptosis, cytotoxicity24 Mar 2014Read
Validation of a Novel Tumoroid-Based Cell Culture Model to Perform 3D in vitro Cell Signaling Analyses in vitro, 3d cell culture, fluorescence based assay4 Dec 2013Read
Live Cell Biosensor Assay Used To Interrogate GPCRs GPCR, luminescence21 Nov 2013Read

Popular tags in SelectScience