Cellular Analysis of 3D Spheroid-Based Tumor Invasion Assays
7 October 2015

This application note demonstrates a method for the generation of 3D spheroidal tumoroid structures, creation of a suitable invasion matrix, image-based monitoring of tumor invasion, and cellular analysis of captured images. Metastasis is the main cause of death in cancer patients and one of the most complex biological processes in human diseases. The development of therapies designed to forestall the metastatic activity of tumors has been met with multiple challenges. Tumors in vivo exist as a 3D mass of multiple cell types, including cancer and stromal cells . Therefore, incorporating a 3D spheroid-type cellular structure that includes co-cultured cell types forming a tumoroid, provides a more predictive model than the use of individual cancer cells cultured on the bottom of a well in traditional 2D format.