Application Note: ZEISS Lightsheet Z.1 Sample Preparation
26 September 2017

This paper describes theoretical and practical aspects of sample preparation for light sheet fluorescence microscopy (LSFM). It presents general rules for sample handling and mounting, as well as guidelines with respect to the best preparative technique to use, taking into account sample type, structure and properties. Step-by-step protocols and recommended materials for ZEISS Lightsheet Z.1 samples are included. These protocols cover sample preparation ranging from micrometer-sized fluorescent beads to millimeter-sized insects, providing detailed information relating to preparation and observation techniques. Finally, this paper identifies the main artifacts and problems that can result from the preparation techniques.