Application Note: Validation of the Activity of G-Protein-Coupled Receptors (GPCRs) Using SPRi
9 January 2018

G-Protein coupled receptors (GPCRs) are a large family of membrane proteins with integral functions in most physiological processes. There are several challenges, however, associated with finding suitable GPCR expression systems and measuring their interactions with ligands at binding sites. Here, Horiba discusses surface plasmon resonance imaging (SPRi) as a technology that facilitates the study of GPCR-ligand interactions and the binding site itself, by covalently immobilizing proteoliposomes on a SPRi-BiochipTM.