Application Note: Ultrafast Analysis of Metabolic Stability Assays Using Agilent RapidFire High-resolution MS
5 November 2012
The metabolic half-life or stability of a drug discovery compound has important pharmacokinetic and clinical significance, because it influences both oral bioavailability and plasma concentration of a compound, ultimately affecting efficacy. Large compound libraries and advancements in liquid handling have placed demands on the throughput of in vitro metabolic stability assays. In this application note the results of a metabolic stability assay generated by LC/MS/MS were compared with those of the Agilent RapidFire System. The results were equivalent, but the RapidFire 360 offered a more efficient workflow and higher throughput. The results indicate that the MRM method development can be eliminated for the metabolic stability assay.