Ultra-Sensitive Host Cell Protein Detection Using CESI-MS with SWATH® Acquisition
19 Jun 2017Host cell proteins (HCPs) are undesired impurities in biologic preparation processes and can negatively affect biotherapeutic quality, potency, and safety. The integration of capillary electrophoresis (CE) and electrospray ionization (ESI) into one process (CESI) presents the possibility to improve the sensitivity of HCP quantitation through reduced ion suppression and improved ionization efficiency at ultralow nanoliter per minute flow rates. This application note demonstrates the use of CESI-MS with SWATH acquisition for the ultrasensitive detection of HCPs in a representative mAb preparation.
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CESI 8000 Plus system
SCIEXExtending the reach of your mass spectrometer. Mass spectrometry (MS) has become an indispensable technology for the analysis of compounds of biological interest. Our improvements in assay sensitivity and reduction in ion suppression reveal new information that is critical to your research, whether you’re characterizing a therapeutic protein, identifying the proteins that make up a specific proteome, analyzing post-transitional modifications, or studying a metabolomic fingerprint. We developed a new front-end separation and ionization technology called CESI, which combines the high efficiency and ultra-low flow of capillary electrophoresis (CE) with an integrated electrospray ionization source. This optimization of electrospray ionization (ESI) into the nanoflow region has been shown to greatly improve assay sensitivity and reduce ion suppression.