Application Note: Solution for Effective Removal of Cell Debris and Subsequent Purification of Biomolecules
15 March 2013

This technical note by Pall Life Sciences presents a solution for the most problematic step in the purification of biomolecules which is the clarification of the sample once the cells have been lysed. In the past, centrifugation was the primary method used to sediment cellular debris, this method has several limitations. However, Pall Life Sciences present a lysate sample preparation procedure using the AcroPrep™ Advance filter plate for lysate clearance that effectively removes unwanted cellular debris from samples. The filter plate provides maximum biomolecule recovery and effectively removes cellular debris from samples prior to purification.