Application Note: Snapshot of MAP Kinase and Related Signal Transduction Pathways from Biopsied Cells Using a Novel Non-Optical Assay Technology
24 September 2012
Aberrant activity of the MAP and PI3 kinases is implicated in many forms of cancer. The ability to gather quantifiable information on the activation state of these kinases from limited in-vitro and in-vivo tumor samples would accelerate drug development and ultimately, the treatment of cancer. In this BioScale poster, presented at ELRIG Drug Discovery 2012, non-optical acoustic membrane microparticle (AMMP®) technology was used to quantitate the activity state of multiple kinases including EGFR, MEK, ERK, AKT, p38 and JNK in a single assay plate. Using fewer than 2000 cells per assessment, multiple kinases were measured, in their native states, in a single assay using AMMP technology.