Sensitive Fluorescence Analysis of Aflatoxins Using Post-Column UV Derivatization on the Agilent 1260 Infinity LC
24 Nov 2012
Aflatoxins are toxic and carcinogenic compounds produced as metabolites by many Aspergillus species. The analysis of aflatoxins in food matrices such as grain and nuts is an important task for the food industry to ensure product safety. This application note from Agilent Technologies presents the analysis of four major aflatoxins (B1, B2, G1 and G2) after sample preparation using an immunoaffinity column, post-column UV derivatization, and fluorescence detection. It is shown that the LOD and LOQ achieved with this method are well below the limits required by the regulatory agencies. The method offers a less hazardous alternative compared to the well-established, post-column electrochemical derivatization method.