Application Note: Rapid Sensitive and Cost-Effective Detection of B Vitamins in Foods by UHPLC/MS/MS
22 November 2012

The B vitamins play important roles in cell metabolism and in promoting human health. They are water soluble and not stored very well in the body, necessitating daily intake through diet or nutritional supplements. While most of the B vitamins are not harmful when ingested in high amounts, B3 and B6 can, in fact be toxic at high concentrations, leading to neuropathy and excessive blood vessel dilation. In this application note a fast UHPLC/MS/MS method, using the Agilent 1290 Infinity LC System coupled to the Agilent 6460 Triple Quadrupole LC/MS System with Agilent Jet Stream technology, is described for the simultaneous extraction and determination of five B vitamins in foods.