Application Note: Rapid, Reliable Measurement of Cellular Proliferation and Toxic Compound Effects on 3D Spheroid Cultures Grown from Multiple Cancer Cell Lines with ATPlite™ 3D and ATPlite 1step 3D
18 May 2017

Cellular viability and toxicity assays are fundamental tools in the drug discovery process – used to evaluate both the potency of compounds, as well as their toxicity profiles for early drug safety assessment. Cells cultured in 3D microtissues exhibit inherent differences in cellular adhesion and require substantially stronger lysis conditions than is sufficient for traditional monolayer or suspension cultures to access the cells deeper inside each spheroid.  In order to address this need, we developed ATPlite 3D and ATPlite 1step 3D assay kits specifically for use with 3D cell cultures.