Application Note: PCR-RFLP Directly from Blood Using Thermo Scientific Phusion Blood Direct PCR Kit
5 November 2013

This application protocol describes how PCR-RFLP (restriction fragment length polymorphism) assays can be performed from blood samples without DNA purification. The approach discussed allows for amplification of DNA directly from various starting materials such as blood, mouse ear and tail tissues, plants, and FFPE tissue samples; allowing for genotyping without DNA purification before PCR or digestion.

Download PDF >>PDF Icon

Read the full article...

  Become a member
  • Quick and complete access to all application articles

  • Download 10,000+
    applications and methods

  • Make informed buying decisions

Already a member?

Log in for full access to the article

Remember me