PCR-RFLP Directly from Blood Using Thermo Scientific Phusion Blood Direct PCR Kit
5 Nov 2013This application protocol describes how PCR-RFLP (restriction fragment length polymorphism) assays can be performed from blood samples without DNA purification. The approach discussed allows for amplification of DNA directly from various starting materials such as blood, mouse ear and tail tissues, plants, and FFPE tissue samples; allowing for genotyping without DNA purification before PCR or digestion.
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Phusion Blood Direct PCR Kit
Thermo Fisher ScientificThermo Scientific Phusion Blood Direct PCR Kit is designed for amplification of DNA from whole blood. It eliminates the need for a separate DNA purification step prior to PCR. The modified Phusion Hot Start II High-Fidelity DNA Polymerase is resistant to PCR inhibitors present in blood and retains its activity even with samples containing 40% whole blood. This extremely accurate proofreading DNA polymerase makes the Phusion Blood Direct PCR Kit an ideal choice even for demanding applications, such as cloning. Phusion Blood Direct PCR Kit also allows analysis of blood samples with different anticoagulants, storage conditions and from various species.Phusion Blood Direct PCR Kit Features: No need for time-consuming and expensive DNA purification steps. Simple protocol requires minimal hands-on time. Extremely short PCR protocol times. Modified Phusion Hot Start II High-Fidelity DNA Polymerase delivers abundant yields of specific product. Phusion Blood Direct PCR Kit Includes: Phusion Blood II DNA Polymerase. 2X Phusion Blood PCR Buffer (includes dNTPs and MgCl2). 50 mM EDTA. 50 mM MgCl2. DMSO. Universal Control Primer Mix.