Application Note: Optimizing Real-time Quantitative PCR Experiments with the Agilent 2100 Bioanalyzer
15 July 2013

Real-time quantitative PCR (QPCR) is a highly sensitive method to assess gene expression changes in biological systems. As for all experimental designs, high quality starting material is essential for the success of the experiment. In this application note read how the Agilent 2100 bioanalyzer can be used to assess the level of degradation of the RNA starting material. In addition, to overcome the limitations of a SYBR Green based melt curve analysis, sizes and purity of all amplicons were assessed with the DNA 1000 assay on the Agilent 2100 bioanalyzer.