Multiplex Measurement of Rat Kidney Cytokines in Serum and Urine using the Fluorokine® MAP

6 Aug 2012

This application note investigates kidney toxicity protein markers by measuring TIM-1/KIM-1/HAVCR, FABP1/L-FABP, Lipocalin-2/NGAL, Cystatin C, and Osteopontin in serum and urine from normal (Sprague Dawley®) and PCK rats using the Fluorokine® MAP Rat Kidney Toxicity Panel.

Fluorokine® Multianalyte Profiling (MAP) Kits

Bio-Techne

Fluorokine MAP Kits for the Luminex platform are highly efficient multiplex assays that have been validated for the quantification of factors in complex matrices and require little or no optimization. For a wider selection of analytes, please see our VersaMAP™ line of development kits validated for use with cell culture supernatants. Features Simultaneously detect multiple analytes Requires small sample volume (Undergoes similar validation testing as the Quantikine® single analyte assays Complete, ready-to-use kits Available in three formats. For details, see Which Bead-based Multiplex Kit is right for me? 1. Custom Premix Kits • Custom ordering tool is used to select the analytes of interest and to submit your order • User defines up to 10 analytes of interest • Bead and Detection Antibody cocktails are sent premixed in respective vials • Quick turn-around time: only 2 - 4 days Please allow additional time when placing orders through a distributor. 2. Custom Base Kits & Bead Sets • Base Kit and Bead Sets are provided separately and mixed by the end-user • Customized for the detection up to 22 analytes, depending on the Base Kit selected • Rapid delivery: typically next day Please allow additional time when placing orders through a distributor. 3. Predefined Kits • Panels are predefined by R&D Systems • Complete, ready-to-use kits • Bead and Detection Antibody cocktails are provided premixed in respective vials • Rapid Delivery: Typically available next day Please allow additional time when placing orders through a distributor. Kit Contents for Fluorokine MAP Products Custom Premix & Predefined Fluorokine MAP Kits • Premixed antibody-coated bead cocktail • Premixed biotinylated detection antibody cocktail • Standard Cocktail(s) • Bead Diluent • Biotin Antibody Diluent • Calibrator Diluent(s) • Streptavidin-PE • Wash Buffer Concentrate • Mixing Bottles • Filter-bottom Microplate • Foil Plate Sealers Base Kits and Bead Sets Base Kit: • Standard Cocktail(s) • Bead Diluent • Biotin Antibody Diluent • Calibrator Diluent(s) • Streptavidin-PE • Wash Buffer Concentrate • Mixing Bottles • Filter-bottom Microplate • Foil Plate Sealers Bead Set: • Antibody-coated beads • Biotinylated detection antibody Luminex is a registered trademark of Luminex Corporation

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Multiplex Measurement of Rat Kidney Cytokines in Serum and Urine using the Fluorokine® MAP