Application Note: Multiplex Detection of Stem Cell Pluripotency Markers in Reference Cancer Cell Lines
22 June 2013
Several proteins, including Oct 3/4, Sox2, KLF4 and Nanog, play a critical role in the maintenance of pluripotency in stem cells. Overexpression of one or a combination of these factors has been reported in solid tumors from a variety of tissues. The presence of pseudogenes and differentially expressed protein isoforms complicates the accurate detection of the physiologically relevant forms of these proteins in cancer stem cell research. In this poster read about the development of MILLIPLEX® MAP multiplex assays for the simultaneous, specific and sensitive detection of the pluripotency markers Oct 3/4, Sox2, KLF4 and Nanog as well as several other stem cell-relevant proteins in cellular lysates.