Application Note: Multiple Methods for Detecting Apoptosis on the BD Accuri C6 Flow Cytometer
29 October 2012
Flow cytometry is a particularly powerful method for detecting apoptosis as it enables researches to gain quantitative data on both apoptotic and dead cells within whole populations and cell subsets. This application note demonstrates why the BD Accuri™ C6 personal flow cytometer is well suited to the study of apoptosis. With the ability to detect four fluorochromes in addition to forward and side scatter, the instrument can perform most flow cytometric apoptosis assays, including Annexin V, caspase activation, PARP cleavage, mitochondrial change, and DNA fragmentation. This application note presents examples of several popular BD Biosciences apoptosis kits run on the BD Accuri C6 and includes suggestions to optimize results.