Multiparametric Analysis of Microalgae for Biofuels Using Flow Cytometry
1 Nov 2013
Photosynthetic microalgae have received significant attention for their potential as a biomass source for biofuels, leading to an explosion in studies of algal growth and lipid biogenesis. This white paper from BD Biosciences explores the opportunities and challenges involved in applying flow cytometry to microalgal biofuel research and development. The BD Accuri™ C6 personal flow cytometer is shown to be suitable for analyzing microalgae of varying sizes, detecting chlorophyll autofluorescence over a wide dynamic range, measuring lipid content using fluorescent stains such as Nile Red and BODIPY 505/515, accurately counting cells, and augmenting throughput via automation.