Modeling Cardiac Hypertrophy: Endothelin-1 Induction with qRT-PCR Analysis
15 Jul 2013
This Application Protocol describes how to utilize iCell Cardiomyocytes in a 5-day post-plating in vitro cellular model of cardiac hypertrophy that is induced by endothelin-1 (ET-1) and quantified via increased NPPB expression using quantitative real-time PCR (qRT-PCR). This protocol should serve as a guide for detecting the regulated expression of other fetal genes (e.g. NPPA) in iCell Cardiomyocytes.