Application Note: Isolation of Total Nucleic Acids from FFPE Tissues using FormaPure DNA
26 May 2017

Extensive archives of formalin-fixed, paraffin-embedded (FFPE) tissues combined with next-generation sequencing (NGS) capabilities provide rich resources for identifying and validating new biomarkers. This application note describes a highly efficient and simple process for isolating both DNA and RNA from FFPE samples using Beckman Coulter’s FormaPure DNA kit. The application note demonstrates that the isolated RNA and DNA have superior functionality, allowing them to be used in retrospective studies of diseased tissue at both the genomic and transcriptomic levels.