Intelligent Use of Retention Time During Multiple Reaction Monitoring for Faster and Extended Compound Screening with Higher Sensitivity and Better Reproducibility

LC-MS/MS instruments operating in Multiple Reaction Monitoring (MRM) are widely used for targeted quantitation and screening on triple quadrupole and hybrid triple quadrupole linear ion trap (QTRAP^® ) systems because of their well known selectivity and sensitivity. However, the current limit of a few hundred transitions per chromatographic run limits the number of analytes that can be monitored per injection. In this application note, find out how the new Scheduled MRM™ Algorithm offered in the Analyst^® software version 1.5, only monitors the MRM transitions of the targeted analytes around the expected retention time.