Genomic DNA Isolation from Whole Blood using AcroPrep™ Advance 96-Well DNA Binding Plates on an Automated Liquid-Handling Robot

12 Apr 2012

The protocol in this note represents an example of a fully automated process for high throughput sample preparation from human blood (anticoagulated) optimized for DNA binding plates, and using commercially available buffers with a liquid handling system.

AcroPrep™ Advance Filter Plates

Pall Life Sciences - Laboratory, Food, Beverage

  AcroPrep™ Advance filter plates are the newest edition to Pall’s filter plate family. New sealing technology and advanced tip design make these the first choice in critical sample prep applications.   Aplications: Lysate Clearance DNA purification Protein purification Multiplex assays Aqueous filtration Particulate removal Size exclusion Bead-based assays Neonatal screening Flow cytometry Solvent filtration Concentration, purification and desalting of proteins and nucleic acids    Features: Smooth well design – Provides consistency in filtration times, as well as efficient sample and bead recovery Optimized outlet tips – Minimizes sample leakage during incubation steps and reduces the presence of hanging drops following filtration New well geometry – Results in faster, more uniform filtration rates across the plate, thereby improving well-to-well, plate-to-plate and lot-to-lot consistency 100% visual inspection – Ensures each well has proper membrane placement and is free from debris Automation compatible – Manufactured in accordance with SBS guidelines, allowing plates to be run in manual, semi-automated and automated processes  

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AcroPrep™ Advance 96-Well Filter Plates for DNA Purification

Pall Life Sciences - Laboratory, Food, Beverage

Maximum Yields of High Quality DNA. Optimized for maximum binding yield of DNA from a variety of sample types New well geometry results in reduced hold-up volume and higher recovery of DNA New outlet tip geometry provides direct flow of samples into receiver plate without concerns of crosscontamination Enhanced membrane ensures high yields of contaminant-free DNA for downstream applications Manufactured in accordance with SBS guidelines, allowing entire DNA purification process to be performed on automated equipment

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Genomic DNA Isolation from Whole Blood using AcroPrep™ Advance 96-Well DNA Binding Plates on an Automated Liquid-Handling Robot