Gene, Transcription Start Site and Splice Site Identification Increased Through Use of Automated Preparative Gel Electrophoresis
9 Sept 2013Gel purification of cDNA and RNA-Seq libraries is the gold standard for purification options but these methods are time consuming and so most protocols have standardized on bead or column purification methods. This scientific poster compares two systems for library purification steps in NGS. The Pippin Prep, a preparative gel electrophoresis system, is shown to offer several advantages over other protocols for this application including efficient removal of low molecular weight library contaminants (primer/adapter artifacts) and accurate size-selection of the library.
Related Products
Request Quote for All Products
Pippin Prep DNA Size Selection System
Sage ScienceThe Pippin Prep is a preparative electrophoresis platform for collection of size-selected DNA samples. The system automates DNA size selection using disposable, pre-cast agarose cassettes to extract fractions according to user-defined software input. Samples are electro-eluted from agarose and collected in buffer. The platform is used for the construction of next-gen sequencing libraries. The system collects fragments (depending on gel type) between 90 bp- 8kb. A higher quality of sample can be extracted, more reproducibly and with no cross-contamination, when compared to manual gel purification methods.