Application Note: Gene, Transcription Start Site and Splice Site Identification Increased Through Use of Automated Preparative Gel Electrophoresis
10 September 2013
Gel purification of cDNA and RNA-Seq libraries is the gold standard for purification options but these methods are time consuming and so most protocols have standardized on bead or column purification methods. This scientific poster compares two systems for library purification steps in NGS. The Pippin Prep, a preparative gel electrophoresis system, is shown to offer several advantages over other protocols for this application including efficient removal of low molecular weight library contaminants (primer/adapter artifacts) and accurate size-selection of the library.