Application Note: Factors Influencing Multiplex Real-Time PCR
9 January 2013

Multiplex PCR is the simultaneous amplification of more than one target sequence in a single reaction. Specifically, duplex PCR is the amplification of two target sequences in one reaction, triplex PCR is the amplification of three targets, and so on. This application note outlines the optimization and validation of duplex PCR and provides recommendations for multiplex reactions with a greater number of targets.