Enhanced Detection of Host-Cell Proteins in Biotherapeutic Preparations using Preparative Electrophoresis Followed by LC–Ion Mobility-MS
Enhanced Detection of Host-Cell Proteins in Biotherapeutic Preparations using Preparative Electrophoresis Followed by LC–Ion Mobility-MS
29 Jun 2015This application note explains the fractionation of Host-cell proteins (HCPs) away from biotherapeutic protein using an off-line preparative electrophoresis device, allowing for sub-ppm level HCP detection. HCPs are proteins produced by the host cells during recombinant protein drug production and can contaminate the therapeutic drug protein even at very low levels. Detection of HCPs was improved through fractionating both the intact and reduced monoclonal antibody.
Related Products
Request Quote for All Products
MALDI SYNAPT G2-Si High Definition Mass Spectrometry
WatersRedefining Resolution - Resolution in 3 Dimensions. MALDI SYNAPT G2-Si is a uniquely powerful and versatile MS platform. It's the only way to combine multiple dimensions of separation for analysis of complex samples, from imaging to biological research to chemical materials characterization. The MALDI SYNAPT G2-Si gives you high performance, accurate mass MALDI MS/MS, superior post-ionization separation with T-Wave IMS, High Definition Imaging (HDI) MALDI workflows, versatile experimental options, and the ultimate UPLC/MS/MS performance.
SageELF Sample Fractionator for DNA and Protein
Sage ScienceThe SageELF is an automated, whole-sample fractionation system for NGS sample prep. SageELF, a whole-sample fractionation tool, is now shipping from Sage Science, developer of the Pippin automated DNA size selection system. The SageELF (short for Electrophoretic Lateral Fractionator) generates 12 contiguous fractions from a whole DNA sample, allowing scientists to generate libraries with multiple insert sizes from the same sample, which has applications in genome sequencing, structural variant detection, and identification of rare splice variants. This sample prep instrument will also be especially useful for preserving precious samples. Users simply load their sample, start the run, and pipette out the fractions later. Unlike in-gel digestion, in which some DNA fragments never leave the gel, the whole DNA sample is electrophoresed into the elution modules.Sage Science targets labor-intensive steps in the sample prep process where automation can offer improved precision and reproducibility. Precise size selection is critical for optimizing sequencing efficiency, improving genome assemblies, and reducing NGS project costs.Features: Make libraries with multiple insert sizes from the same sample Ideal for rare or precious samples Automated for precise, reproducible results Applications: Genome sequencing Structural variant detection Rare splice variant detection
