Dual Extraction of RNA and Protein from Skin
10 Jan 2012
Human skin is easily accessible and therefore obtainable as a simple clinic biopsy sample, but is difficult to process owing to its natural resistance to mechanical shearing and high levels of RNases and proteases. A method has been developed for the sequential extraction of high quality RNA and proteins from a single 3 mm full thickness skin biopsy with the FastPrep® System. This method yields 1–2 µg of RNA and 150 µg of protein, which is usable in many sensitive downstream applications including microarray, quantitative real-time PCR, two-dimensional gel electrophoresis and Western blot analysis.