Dose Response of Effect of Levocetirizine on Eosinophil Adhesion

22 Oct 2012

In this application note the effect of levocetirizine on human eosinophil adhesion to VCAM-1 and BSA is assessed. Using Cellix’s biochips and Mirus pumping system, eosinophil adhesion to rhVCAM-1 and the dose response effect of levocetirizine on GM-CSF-stimulated eosinophil adhesion to rhVCAM-1 under physiological flow conditions is investigated.

Vena8 Endothelial+ Biochip: microfluidic flow chamber

Cellix Ltd

The Vena8 Endothelial+™ biochip is ideal for studying cell-cell interaction. Endothelial cells are easily seeded (via standard pipette) and cultured inside the microcapillaries of the biochip. As cells flow through the microcapillary of the Vena8 Endothelial+™ biochip, the cell surface receptors may interact with the endothelial cell monolayer which coats the microcapillary walls. Ideal for use with phase contrast imaging and fluorescence imaging to study cell-cell interactions under conditions mimicking physiological flow.

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Vena8 Fluoro+ Biochip: microfluidic flow chamber

Cellix Ltd

The Vena8 Fluoro+™ biochip is ideal for studying cell receptor-ligand interaction and is compatible with confocal microscopy. As cells flow through the microcapillary of the Vena8 Fluoro+™ biochip, the cell surface receptors may interact with adhesion molecules or ligands which coat the microcapillary walls. Ideal for use with fluorescence immunostaining, confocal microscopy and for applications such as thrombosis.

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Dose Response of Effect of Levocetirizine on Eosinophil Adhesion