ResourceForensics
DNA Extraction From Hair Using Pressure Cycling Technology (PCT)
30 Jul 2012Current methods for extracting DNA from hair shafts involve extensive sample preparation and
processing, and often require the use of hazardous chemicals. Even though most hair extraction
methods include a post extraction clean-up or concentration step, PCR inhibitors may still be present
and interfere with downstream sample processing and analyses. A technology novel to the forensic
community, i.e., pressure cycling, has the potential to enhance current DNA extraction methods by
increasing DNA recovery while preserving the quality of the DNA.
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DNA / RNA Extraction and PurificationPurified DNA and RNA are required for numerous downstream molecular biology applications. Consequently, the importance of high-quality DNA/RNA extraction and purification equipment cannot be underestimated. Many purification kits are available and are typically optimized for nucleic acid type and source, including plasmid DNA, genomic DNA, mRNA, RNA and viral nucleic acid purification kits. Automated extraction and purification of nucleic acids can be implemented with magnetic bead separator instruments or high-throughput purification workstations. Find the best DNA/RNA extraction and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.DNA SequencingDNA sequencing, such as sanger sequencing, is a biological technique that determines the precise order of nucleotide bases in a fragment or template of DNA. DNA sequencers and genetic analyzers are based on capillary electrophoresis, where labeled DNA fragments are electrophoretically separated by size as they migrate through a polymer. Find the best DNA sequencing products, including DNA sequencing kits, genomic libraries and genetic identity kits in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.PCT