Determination of Mycotoxin Residues by LC-MS/MS Featuring Two Alternate Sample Extraction Procedures

20 Apr 2016

Mycotoxins are toxic natural secondary metabolites and are produced by many species of fungi, while over 300 mycotoxins have been identified only a few of these pose a significant food or animal feed safety risk. They are still of concern however as they cannot be destroyed during food processing and heat treatment, therefore it is important that food can be analyzed to detect their presence. This application note describes a method to detect mycotoxins using LC-MS/MS with two different sample preparation steps.

SELECTRA® HPLC Columns

UCT, Inc.

High purity, Type B, spherical silica support. 100A pore size. 1.8um, 3um, & 5um partical sizes. Variety of stationary phases.

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ChloroFiltr® for QuEChERS dSPE Clean-Up

UCT, Inc.

Chlorophyll in QuEChERS extracts must be removed to reduce potential instrument downtime. This is removed from extracts using graphitized carbon black (GCB) Unfortunately, GCB also removes planar analytes reducing analytical accuracy. ChloroFiltr®, used in place of GCB in dSPE tubes, removes unwanted chlorophyll without removing pesticides or environmental analytes. No change in procedure are required.

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