Application Note: CRISPR/Cas9 Genome-Wide gRNA Library for Target Identification
7 June 2017

Genome-wide loss-of-function screening is a fundamental method to identify genes responsible for driving biological responses, and complex pooled lentiviral-based libraries expressing large numbers of genetic disruptors, such as shRNAs, make large-scale cell screening practical. This study investigates the potential differences in the RNAi and CRISPR HTGS technologies, by performing parallel dropout viability screens using module-1 human genome-wide CRISPR sgRNA library and module-1 human genome-wide RNAi shRNA library, both modules targeting the same set of 6,300 genes with the same number of effectors (sgRNA or shRNA) for each gene.

Company