Automation of Agencourt AMPure Purification Kit for the Purification of Next-Generation Sequencing Sample Preparation Reactions on the Agilent Bravo Automated Liquid Handling Platform
24 Apr 2013
This application note demonstrates a new method to automate library construction for the Illumina sequencing platform using the Agilent Bravo Automated Liquid Handling Platform and the Agencourt Ampure PCR purification kit. Using the Agilent VWorks Automation Control software, a protocol was developed for the Bravo platform to aspirate and dispense enzyme master-mixes, and perform cleanups of these reactions through the transfer of the Agencourt AMPure reagent to the reaction, transfer of the microtiter plate to a magnet using the integrated gripper, and subsequent sample wash and elution steps. Sample quality and quantity were assessed using the Agilent Bioanalyzer 2100 and Illumina Genome Analyzer IIX.