Automated miRNA Isolation Using Agencourt SPRI and Biomek
17 Dec 2012
This application note describes an efficient and robust miRNA extraction method using Agencourt SPRI (Solid Phase Reverse Immobilization) technology and Biomek automation from Beckman Coulter. The SPRI procedure is an easy, rapid, high yield, and automation-friendly nucleic acid purification method that does not require organic solvents, centrifugation or filtration steps. The presented method provides the capability to compare miRNA and gene expression profiles within the same sample, which allows users to save time and cost for sample preparation.