ResourceLab Automation
Automated Compound Profiling Applications for the Assessment of Lead Compound Off-target Effects
22 Mar 2012Profiling lead compounds against other enzymes within the same target class has become increasingly important in the drug discovery process. Drugs must demonstrate specificity for the target enzyme, or run the risk of generating adverse health effects among patients. This process involves generating IC50 or EC50M values with a variety of enzymes in order to determine specificity. In this application note profiling applications for two important drug target classes, kinases and histone deacetylases, using an 8-channel liquid handler and hybrid multi-mode microplate reader are demonstrated. Assay optimization and profiling data demonstrate the validity of each automated process.
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Combinatorial ChemistryCombinatorial chemistry, also known as combichem, is a technique used in drug discovery to create libraries of structurally related compounds. A library is generated by synthesis with a chemical reactor system or by computer-based modeling of compound combinations. When undertaking combinatorial chemistry consider reagents, buffers, resins and standards.Compound LibrariesCompound libraries, or chemical libraries, are used in drug discovery for the identification of potential therapeutics compounds. Used in conjunction with high-throughput screening, the libraries of stored compounds are often generated for specific purposes as a drug target or disease model. Cheminformatics are commonly used when designing a compound library and software can be used to analyze the screening process. Biopharmaceutical AdvancesBiopharmaceutical advances follow the development of pharmaceuticals derived from biotechnology, also known as biotechnology medicines. Biopharmaceuticals may be produced from cell lines, plants, or microbial cells. Important considerations of biopharmaceutical use include application, cost, production process and purification.Automated Liquid HandlingAutomated liquid handling equipment is used to mix, dilute and dispense allotted volumes of liquid into microplates and other vessels automatically. The robotic, liquid handling arms can dispense from single channel to 3456 multichannel as well as operate nanoliter dispensing, enabling high throughput of samples. Find the best automated liquid handlers in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Compound Profiling