Application Note: Automated Colony Counting for Molecular Biologists
21 June 2013

Transformation efficiency is a measure of the amount of cells within the bacterial culture that are able to take up DNA molecules. Some applications such as the construction of genomic libraries require that the bacteria have a high transformation efficiency. Counting of recombinant colonies is one criteria used to calculate transformation efficiency. In this application note read how ACOLyte and ProtoCOl systems allow rapid automated counting of antibiotic resistant recombinant colonies on spread or spiral plates, as well as colonies and plaques on pour plates.