A Multiplexed Cell-Based Assay for Antibody Screening Against Cell Surface Receptors>

Application Articles

A Multiplexed Cell-Based Assay for Antibody Screening Against Cell Surface Receptors
12 Mar 2013

This application note describes the use of TTP Labtech’s mirrorball for the determination of antibody binding to human epidermal growth factor receptor (EGFR) using a “mix-and-read” assay as an example of a screen for a cell surface antigen. It is shown that mirrorball is capable of distinguishing antibody binding in more than one cell line in a single well. This ability to multiplex reduces costs, increases throughput and eliminates intraplate variability.