Application Note: A Mix-and-Read Cell-Based Assay for Antibody Screening Against Epidermal Growth Factor Receptor
20 March 2013

This poster describes the development of mix-and-read methods for the screening of antibodies against cell surface proteins expressed on live cells. These methods were used to determine human epidermal growth factor receptor (EGFR) antibody binding in A549 cells. Results demonstrate use of the Mirrorball microplate cytometer (TTP Labtech) for the EGFR binding assay proved straightforward.