A Flexible and Robust Solution for Automated 384-well HDAC Profiling
6 Feb 2012
A Flexible and Robust Solution for Automated 384-well HDAC PrHistone deacetylases (HDACs) remove acetyl groups from ε-N-acetyl lysine residues on histones and other proteins. Acetyl group removal serves to increase the positive charge of histone tails, thus encouraging binding between histones and the DNA backbone and preventing transcription. HDACs are implicated in many human diseases, including cancer; making them an increasingly popular drug discovery research target. In this application note BioTek monitors the deacetylation activity of multiple Class I and II HDAC enzymes in an automated fashion. Validation and pharmacology results demonstrate how the fluorescent assay technology and automated instrumentation may be used together to assess enzyme kinetics and inhibition.