Automation of Sample Preparation for Accurate and Scalable Quantification and Characterization of Biotherapeutic Proteins Using the Agilent AssayMAP Bravo Platform
13 October 2015
An ever-increasing number of potential biotherapeutic proteins must be rapidly and accurately characterized so data-driven decisions can be made to move the best candidates efficiently through the drug discovery and development process. This has resulted in a sample processing bottleneck that demands more rapid, scalable, and reproducible methods to supplant current low-throughput and laborious manual sample prep methods for common workflows such as protein purification and quantification, whole protein mass determination, sequence coverage analysis, and characterization of post translational modifications (PTMs). This application note demonstrates the utility of the platform for widely-used bioprocess workflows and benchmarks analytical figures-of-merit for automated antibody purification.