SuperScript IV Reverse Transcriptase
1 September 2015
Reverse transcriptases are a class of enzymes that synthesizes cDNA from a RNA template. Traditionally, scientists used reverse transcriptases to clone genes and study expressed genes from whole organisms or cells. However, the scientific landscape has drastically changed to include single-cell analysis, sample preparation–free analysis, and next-generation sequencing, requiring new reverse transcriptases with single-copy sensitivity, speed, resilience to difficult samples, and thermoreactivity. This white paper demonstrates the use of SuperScript IV RT in the presence of a variety of inhibitors, such as alcohols, salts, detergents, heparin, hematin, bile salts, and formalin, typically found in sample preparation reagents, cell lines, blood, feces, and FFPE samples.