Screening for Use-Dependent Blockers of NaV1.5 Channels on the IonWorks Barracuda Automated Patch Clamp System
18 Nov 2013

This application note describes the design and validation of a cell based assay for the NaV1.5 channel, using the IonWorks Barracuda® Automated Patch Clamp System. A single concentration mock screen of known sodium channel blockers was set up to identify the location of the hits in a 384 compound plate. Follow-up experiments on the hits were then performed to identify use- and state-dependent blockade using a train of depolarizing pulses.

Show    per page
1 of 1     Page  1 Next 
Multiplexing Cell-Based Assays using the SpectraMax® i3 Multi-Mode Microplate Reader with MiniMax™ Imaging Cytometer drug development, cytometry, cell imaging7 Oct 2013Read
Using CombiCult® in combination with ClonePixTM Technology to Select Media Components which Induce MSC Cells to Secrete High Levels of VEGF stem cell research, assay development, screening1 Oct 2013Read
Customised Multi-Parametric Image Analysis For Accelerated Toxicity Screening image analysis, live cell imaging, assay development30 Sep 2013Read
Intrinsic tryptophan detection with the SpectraMax i3 Multi-Mode Microplate fluorescence based assay, protein analysis, protein detection22 Aug 2013Read
Cell Counting and Morphology Visualization Utilizing Microplate Imaging Cytometry cell viability, fluorescence based assay, cell imaging31 Jul 2013Read
Measuring Marker Expression with Imaging Cytometry on a Plate Reader microplate reader software, fluorescence based assay, cell imaging30 Jul 2013Read
Toxicity Assays Using Induced Pluripotent Stem Cell-Derived Cells fluorescence based assay, drug testing, live cell imaging29 Jul 2013Read
High-Content Analysis of the Various Stages of Apoptosis with the MetaXpress Cell Health Application Module apoptosis, cell viability, drug development17 Apr 2013Read

Popular tags in SelectScience