Application Note: ELF from Sage Science: A New Automated System for Size-Selection in NGS Library Construction
9 Feb 2015
Sage Science’s new size selection system, called the ELF (Electrophoretic Lateral Fractionator) is designed to fractionate a single genomic sample into 12 contiguous size fractions. The system uses a two-dimensional process to separate DNA through an agarose column in a first direction, and then move the separated DNA fragments sideways into a linear array of buffer-filled elution modules that are positioned alongside the separation column. The instrument can operate in direct current mode for samples up to mid-single kilobases in size, or in pulsed field mode for samples up to 50 kilobases in size. Fractionation range is controlled by selection of voltage protocol, gel concentration, and run timing. Internal standards can be used to improve run-to-run reproducibility, and the instrument can read fluorescein-labeled markers.