Total Protein Quantification in Presence of Imidazole using Spectral Content Profiling on the Xpose
18 Aug 2014
This spectral profiling application is specifically designed to quantify the amount of protein within a sample in the presence of imidazole. The app can be used to quantify purified proteins or Ni-affinity column fractions. The specific protein profile is then used for determining its concentration. This note describes how to use this application and how to interpret its results.