Antibody Development to Bioproduction Using CHO Transient Gene Expression: Gram/Liter Antibody Titers and Rapid Generation of High Yield, Stable Cell Lines via Flow Electroporation
3 Feb 2015
Flow electroporation technology greatly shortens the timeline of antibody development by enabling large scale transient gene expression (TGE) directly within CHO cells. This poster presents data demonstrating the reproducibility and scalability of MaxCyte flow electroporation as well as its capacity to produce antibody titers >1g/L using TGE. In addition, it shows the use of MaxCyte electroporation for creation of stable CHO pools and the rapid generation of high-yield stable cell lines within 6-8 weeks of transfection.