Screening of GPCR-Antagonists Using the Calcium Mobilization Assay
19 July 2017

This application note describes a study in which three compounds were screened for P2Y2 receptor inhibition using a calcium mobilization assay with two different fluorescent dyes. The fluorescence intensity signal was measured using a TriStar2 LB 942 plate reader. The basic principle behind the assay is described and the materials and methods detailed. It was found that the TriStar2 LB 942 could accurately screen test compounds for antagonist activity with precision and high reproducibility.