cAMP/IP-One HTplex™ Cell-Based Experiment Performed on Mithras LB 940 using HTRF® Technology
26 April 2017

This application note describes the cAMP and IP1 HTplex competitive immunoassay which can detect both cAMP and IP1 simultaneously by using two different acceptors. It describes the assay principle, protocol and results. The Mithras LB 940, a multimode plate reader, is equipped with an additional emission filter to detect acceptor emission. The application note concludes that this assay produces reliable results, as evidenced by the excellent Z values and the comparison of the results to that found in single analyte assays.