The ProteOn™ HTG Sensor Chip: Novel Surface for Stable Capture of Histidine-Tagged Proteins for Protein-Protein Interaction Analysis
27 Jan 2015
The ProteOn HTG chip is a new SPR sensor chip for use with the Bio-Rad ProteOn XPR36 protein interaction array system. In this report, different models are used to show the high stability of surface-bound ligand proteins, surface regeneration for chip reuse, and the ability to capture nonpurified histidine-tagged proteins. The kinetics of protein-protein interactions were analyzed and high quality results were obtained showing the potential applications of the HTG chip, including high-throughput screening for mutagenesis research.