Activity Measurements of Pentose Phosphate Pathway Enzymes Using the Thermo Scientific NanoDrop 2000c Kinetics Application
17 Dec 2014
The method described in this application note demonstrates the ease of performing enzymatic activity measurements by continuous spectrophotometric assays. Here, an enzyme from the pentose phosphate pathway, ribokinase, was characterized. The activity results were in perfect agreement with previous literature and thus attest to the reliability of the NanoDrop 2000c as a kinetics-measuring instrument.