Determination of Trans,Trans-Muconic Acid in Urine Samples
25 Feb 2015
This application note describes a gradient method using a sub-2 μm column for the determination of the benzene metabolite, trans,trans-muconic acid (tt-MA) in urine samples. The separation of the target compound from the its complex urine matrix could be carried out in less than in 6 minutes using the KNAUER PLATINblue UHPLC system. The high speed and reliability of the method make it well-suited for routine analysis in clinical diagnostics. The retention time of tt-MA applying a classical HPLC separation is in the range of approximately 16 min. With the new developed UHPLC method tt-MA elutes after 2.4 min from the column.