On-Bead Antibody-Small Molecule Conjugation and pH Sensor Fluorescent Dye for Screening of Internalizing Antibodies for ADC Applications
1 July 2015

This poster demonstrates a method that allows rapid screening of large number of antibodies for internalization in the cell. The method is a combination of two novel approaches:  first, ‘on-bead’ antibody-small molecule conjugation that uses high capacity magnetic Protein A and Protein G beads to capture the antibody directly from the cell media and conjugate with a reactive small molecule, and second, conjugation of the antibody with a pH sensor dye that becomes fluorescent only when the antibody is internalized and trafficked to the endosomes and lysosomes.