PEGylated Protein and Free PEG Analysis by Size Exclusion Chromatography and Charged Aerosol Detection
30 October 2009

This application note demonstrates size exclusion chromatography (SEC) and Corona Charged Aerosol Detection to resolve both pegylated monoclonal antibody (PEG-MAb) and free PEG, molecules important in drug development and QC. The typical elution order expected for SEC is reversed with this mobile phase due to hydrophobic interactions causing the PEG-MAb to retain longer. This approach can readily detect 0.1% PEG in the presence of the PEG-MAb product.