Determination of Enzyme Kinetics in the Eppendorf BioSpectrometer® kinetic Using a Hexokinase and Glucose-6-Phosphatedehydrogenase from Saccharomyces cerevisiae
19 Aug 2014

This guide demonstrates two applications of measurements of enzyme kinetics using the Eppendorf BioSpectrometer kinetic; enzymatic determination of ATP concentration, and determination of enzyme activities. Learn how important parameters, such as optimal enzyme or substrate concentrations, measurement duration, linear range of the curve or reaction temperature, may be assessed in preliminary experiments from which activity measurements can then be based.

Show    per page
1 of 2     Page  1 2 Next 
Eppendorf Cell Culture Consumables –Improved Optical Performance Facilitates Microscopic Analysis of Cells cellular growth, cell analysis7 Aug 2014Read
Evaluation of Kits for RNA Determination Using the Eppendorf BioSpectrometer® fluorescence molecular biology, fluorescence, RNA quantification4 Jul 2014Read
Comparative Run Time Evaluations of PCR Thermal Cyclers qPCR, Mastercycler Nexus, RT qPCR3 Dec 2013Read
Amino Acid Fermentation: Evaluation of Scale-Down Capabilities Using DASbox® Mini Bioreactors amino acids, fermentation28 Nov 2013Read
Increased Yield with Isolation of a Low-Copy Plasmid Using Eppendorf Tubes® 5.0 mL Compared to the 1.5 mL and 2 mL Formats molecular biology27 Nov 2013Read
ep Dualfilter T.I.P.S. SealMax – Determination of Sample Recovery Rate of Self-Sealing Filter Tips in Case of Over-Pipetting contamination prevention26 Nov 2013Read
Applications of the New Eppendorf 5.0 mL system sample handling, sample storage14 Nov 2013Read
Factor Based Quantification of Nucleic Acid Concentrations in the Eppendorf PlateReader AF2200 nucleic acid concentration6 Nov 2013Read
Imaging of Single Cell Gene Expression via In Situ RT-PCR on the Mastercycler® nexus flat immunohistochemistry, single cell analysis, single cell genomics24 Oct 2013Read
Cultivation of Human CAP® Cells: Evaluation of Scale-Down Capabilities using Single-Use Bioreactors cellular growth, bioprocessing, cell incubation7 Oct 2013Read

Popular tags in SelectScience